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BFGF Antibody / Basic FGF / FGF2

CAT: 0800-V5062-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V5062-20UGSize:20 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10-FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P09038
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 7-96) from the human protein was used as the immunogen for the bFGF antibody.
Clonality
Monoclonal
Isotype
IgG2 κ
Clone
FGF2/7364
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the bFGF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This bFGF antibody is available for research use only.
Storage Conditions
Aliquot the bFGF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the bFGF antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
IHC staining of FFPE human placental tissue with bFGF antibody (clone FGF2/7364) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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