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Gelsolin Antibody

CAT: 0800-R30038Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30038Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL for acetone fixed tissues
Host
Mouse
Reactivity
Human, Rabbit
Immunogen
Human plasma gelsolin was used as the immunogen for this Gelsolin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
GS-2C4
Applications
WB, IHC-P, IHC-F
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Gelsolin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Gelsolin antibody is available for research use only.
Storage Conditions
After reconstitution, the Gelsolin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Gelsolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC testing of FFPE human lung cancer tissue with Gelsolin antibody. HIER: steamed with pH6 citrate buffer, HRP-staining.

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