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CD3 Antibody

CAT: 0800-R30014Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30014Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3) . Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
CAS Number
9007-83-4
Specifications
IHC (Frozen) : 1 µg/mL-formalin or acetone fixed tissues, FACS: 1-3ug/10^6 cells
Host
Mouse
Reactivity
Human
Immunogen
Human thymocytes followed by Sezary T cells was used as the immunogen for this CD3 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
CA-3
Applications
IHC-F, FACS
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the CD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD3 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the CD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Flow cytometry testing of human PBMC with CD3 antibody at 1ug/10^6 cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue=CD3 antibody.

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