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Recombinant ACTA2 Antibody

CAT: 0800-R20272-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20272-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant Alpha Smooth Muscle Actin / ACTA2 antibody reacts to human and mouse alpha smooth muscle Actin (alpha-Actin-2) . It may also react to bovine or rat alpha smooth muscle Actin, as predicted by immunogen homology.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1:1000-1:2500 (1), Western blot: 1:1000-1:2000
UniProt
P62736
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A peptide corresponding to the N-terminus of human alpha smooth muscle Actin was used as the immunogen for this recombinant ACTA2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM253
Type
Recombinant
Applications
WB, IHC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant ACTA2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant ACTA2 antibody is available for research use only.
Storage Conditions
Store the recombinant ACTA2 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Prediction Reactivity
Bovine, Rat
Image Legend
Western blot testing of mouse heart lysate with recombinant ACTA2 antibody at 1:1000. Predicted molecular weight ~42 kDa.

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