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Recombinant CD146 Antibody

CAT: 0800-R20269-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20269-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant CD146 antibody reacts to human CD146 (Cell surface glycoprotein MUC18) . It may also react to the mouse and rat protein, as predicted by immunogen homology.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1:200-1:500 (1), Western blot: 1:1000-1:2000
UniProt
P43121
Host
Rabbit
Reactivity
Human
Immunogen
A peptide corresponding to the residues near C-terminus of human CD146 was used as the immunogen for this recombinant CD146 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM249
Type
Recombinant
Applications
WB, IHC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant CD146 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant CD146 antibody is available for research use only.
Storage Conditions
Store the recombinant CD146 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant CD146 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Prediction Reactivity
Mouse, Rat
Image Legend
Western blot testing of human A375 cell lysate with recombinant CD146 antibody at 1:1000. Observed molecular weight 70-120 kDa depending on glycosylation level.

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