CD146 Antibody

• Description:

  CD146/MUC18/MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. [UniProt]

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  P43121

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for the CD146 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CDLA146

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the CD146 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD146 antibody is available for research use only.

• Storage Conditions:

  Store the CD146 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasm

• Image Legend:

  IHC testing of human melanoma and CD146 antibody (clone CDLA146)