CD146 Antibody

• Description:

  CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE & Frozen) : 1-2 µg/mL

• UniProt:

  P43121

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein (amino acids H59-A401) was used as the immunogen for the CD146 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  2H12

• Applications:

  WB, IHC-P, IHC-F

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CD146 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CD146 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CD146 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CD146 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC staining of frozen human placenta with CD146 antibody.