Recombinant GAPDH Antibody (Loading Control)

• Description:

  This recombinant GAPDH antibody reacts to Glyceraldehyde 3-phosphate dehydrogenase.

• Specifications:

  Western blot: 1:1000, Immunoprecipitation: 1:200, Chromatin IP (ChIP) : 1:200, Immunocytochemistry: 1:200, Immunohistochemistry: 1:200 (1)

• UniProt:

  P04406

• Host:

  Rabbit

• Reactivity:

  All Species Expected

• Immunogen:

  A peptide corresponding to the C-terminus of GAPDH was used as the immunogen for this recombinant GAPDH antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  RM114

• Type:

  Recombinant

• Applications:

  WB, IP, ICC, IHC, ChIP

• Purity:

  Protein A purified from animal origin-free supernatant

• Format:

  Purified

• Buffer:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Reconstitution:

  Store the recombinant GAPDH antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

• Limitations:

  This recombinant GAPDH antibody is available for research use only.

• Storage Conditions:

  Store the recombinant GAPDH antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

• Formulation:

  Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the recombinant GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

• CAS Number:

  9000-83-3

• Image Legend:

  Western blot of human A431 cell lysate using the recombinant GAPDH antibody at 1:1000. Predicted molecular weight ~36 kDa.