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Recombinant GAPDH Antibody (Loading Control)

CAT: 0800-R20198-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20198-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant GAPDH antibody reacts to Glyceraldehyde 3-phosphate dehydrogenase.
CAS Number
9000-83-3
Specifications
Western blot: 1:1000, Immunoprecipitation: 1:200, Chromatin IP (ChIP) : 1:200, Immunocytochemistry: 1:200, Immunohistochemistry: 1:200 (1)
UniProt
P04406
Host
Rabbit
Reactivity
All Species Expected
Immunogen
A peptide corresponding to the C-terminus of GAPDH was used as the immunogen for this recombinant GAPDH antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM114
Type
Recombinant
Applications
WB, IP, ICC, IHC, ChIP
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant GAPDH antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant GAPDH antibody is available for research use only.
Storage Conditions
Store the recombinant GAPDH antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Image Legend
Western blot of human A431 cell lysate using the recombinant GAPDH antibody at 1:1000. Predicted molecular weight ~36 kDa.

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