GAPDH Antibody Loading Control

• Description :

  Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 3-5 µg/mL

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat, Dog

• Immunogen :

  A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  GAP259

• Applications :

  WB, IHC-P

• Purity :

  Protein G Affinity Chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Aliquot and store the loading control antibody frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

• Limitations :

  This GAPDH antibody loading control is available for research use only.

• Storage Conditions :

  Aliquot and store the loading control antibody frozen at -20°C or colder to avoid repeated freeze-thaw cycles.

• Formulation :

  0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, nuclear

• Image Legend :

  Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.