GAPDH Antibody Loading Control

• Description:

  Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 3-5 µg/mL

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  GAP259

• Applications:

  WB, IHC-P

• Purity:

  Protein G Affinity Chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Aliquot and store the loading control antibody frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

• Limitations:

  This GAPDH antibody loading control is available for research use only.

• Storage Conditions:

  Aliquot and store the loading control antibody frozen at -20°C or colder to avoid repeated freeze-thaw cycles.

• Formulation:

  0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.