CLEC4E Antibody

• Description:

  CLEC4 is a member of the C-type lectin family of proteins that plays a role in inflammatory and immune response. CLEC4E, also called Mincle (Macrophage-inducible C-type lectin), is found on the surface of activated macrophages and phagocytes. It is a receptor for both non-self (fungi and mycobacteria), and self (damaged cells) ligands. Dead cells release SAP130 (Sin3A-Associated Protein, 130kDa), a small nuclear ribonucleoprotein component. CLEC4E senses this and interacts with the ITAM-containing adaptor protein FcRγ , and via Syk and CARD9 signaling, activates the production of inflammatory cytokines such as TNFa, MIP-2 and IL-6.

• Specifications:

  Western blot: 2-5 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A recombinant protein fragment from the C-terminal portion of human CLEC4E was used as the immunogen for this antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Aliquot the CLEC4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CLEC4E antibody is available for research use only.

• Storage Conditions:

  Aliquot the CLEC4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Provided assay concentrations are suggestions only, CLEC4E antibody titration may be required for optimal results.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human samples with CLEC4E antibody at 2ug/ml.