Clathrin Light Chain A Antibody / CTLA

• Description:

  Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.

• Specifications:

  Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

• UniProt:

  P09496

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 99-127 from the human protein was used as the immunogen for the Clathrin Light Chain A antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, WB, IHC-P

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Clathrin Light Chain A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Clathrin Light Chain A antibody is available for research use only.

• Storage Conditions:

  Aliquot the Clathrin Light Chain A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Clathrin Light Chain A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of FFPE human lung carcinoma tissue with Clathrin Light Chain A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.