Squalene synthase Antibody / SQS / FDFT1

• Description:

  FDFT1 / Squalene synthase catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.

• Specifications:

  Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

• UniProt:

  P37268

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 140-170 from the human protein was used as the immunogen for the Squalene synthase antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC-P

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Squalene synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Squalene synthase antibody is available for research use only.

• Storage Conditions:

  Aliquot the Squalene synthase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Squalene synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of FFPE human hepatocellular carcinoma tissue with Squalene synthase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.