SOX2 Antibody

• Description :

  SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.

• Specifications :

  Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

• UniProt :

  P48431

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 251-284 from the human protein was used as the immunogen for the SOX2 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  IF, WB, FACS

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This SOX2 antibody is available for research use only.

• Storage Conditions :

  Aliquot the SOX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  The stated application concentrations are suggested starting points. Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  Western blot testing of human NCCIT cell lysate with SOX2 antibody. Predicted molecular weight ~34 kDa.