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SIGLEC5 Antibody

CAT: 0800-F54705-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54705-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25
UniProt
O15389
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 8-39 from the human protein was used as the immunogen for the SIGLEC5 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SIGLEC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SIGLEC5 antibody is available for research use only.
Storage Conditions
Aliquot the SIGLEC5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the SIGLEC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human T-47D cell lysate with SIGLEC5 antibody. Expected molecular weight: 60-110 kDa depending on glycosylation level.

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