CD33 Antibody / SIGLEC3

• Description:

  Recognizes a 67kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P20138

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment of human CD33 protein was used as the immunogen for the CD33 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2 κ

• Clone:

  SIGLEC3/7612

• Applications:

  WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD33 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD33 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD33 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell Surface, Cytoplasm

• Image Legend:

  Western blot testing of human THP-1 cell lysate with CD33 antibody (clone SIGLEC3/7612) . Predicted molecular weight is 40-67 kDa depending on glycosylation level.