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HIST1H2BJ Antibody

CAT: 0800-F54560-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54560-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25
UniProt
P06899
Host
Rabbit
Reactivity
Human, Zebrafish
Immunogen
A portion of amino acids 57-86 from the human protein was used as the immunogen for the HIST1H2BJ antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HIST1H2BJ antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HIST1H2BJ antibody is available for research use only.
Storage Conditions
Aliquot the HIST1H2BJ antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the HIST1H2BJ antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Western blot testing of 1) ZR4, 2) whole zebrafish and 3) zebrafish muscle lysate with HIST1H2BJ antibody. Predicted molecular weight ~14 kDa.

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