Complement factor H Antibody / CFH

• Description:

  This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• Specifications:

  Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25

• UniProt:

  P08603

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 751-780 from the human protein was used as the immunogen for the Complement factor H antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  IHC-P, WB, IF

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Complement factor H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Complement factor H antibody is available for research use only.

• Storage Conditions:

  Aliquot the Complement factor H antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Complement factor H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) lung and 2) plasma lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.