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Vimentin Antibody

CAT: 0800-F54035-0.1MLSize: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0800-F54035-0.1MLSize:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, ICC/IF: 1:800, Immunoprecipitation: suitable
UniProt
P08670
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant partial protein was used as the immunogen for this Vimentin antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
4F8-A5-H5
Applications
WB, ICC/IF, IP
Purity
Protein G affinity
Format
Purified
Buffer
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Reconstitution
Store the Vimentin antibody at -20oC.
Limitations
This Vimentin antibody is available for research use only.
Storage Conditions
Store the Vimentin antibody at -20°C.
Formulation
In PBS with 50% glycerol and 0.03% ProClin 300
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.

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