RAB13 Antibody

• Description :

  The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin- induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.

• Specifications :

  Western blot: 1:500-1000

• UniProt :

  P51153

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A human recombinant protein corresponding to amino acids 1-203 was used as the immunogen for the RAB13 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  1600CT845.37.29

• Applications :

  WB

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the RAB13 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This RAB13 antibody is available for research use only.

• Storage Conditions :

  Aliquot the RAB13 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the RAB13 antibody may be required due to differences in protocols and substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of human 1) MCF7, 2) A431, 3) A549 and 4) U87 MG cell lysate with RAB13 antibody at 1:1000. Predicted molecular weight: 23 kDa.