IRAK4 Antibody

• Description:

  Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

• UniProt:

  Q9NWZ3

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 5-36 from the human protein was used as the immunogen for this IRAK4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the IRAK4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This IRAK4 antibody is available for research use only.

• Storage Conditions:

  Aliquot the IRAK4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the IRAK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Bovine, Mouse

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of IRAK4 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the IRAK4 gene. Predicted molecular weight 52/37 kDa (isoforms 1/2) .