Raptor Antibody

Raptor is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E) . mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. [UniProt]

9007-83-4

IHC (Paraffin) : 1:25, Western blot: 1:500-1:1000

Q8N122

Human, Mouse, Rat

This Raptor antibody was produced from a mouse immunized with recombinant protein.

1411CT316.2.151.34

IHC, WB, ELISA

Purified

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

Aliquot the Raptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

This Raptor antibody is available for research use only.

Aliquot the Raptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

Titration of the RAPTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.