C-Src Antibody

• Description:

  The gene for Src is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. It is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in Src could be involved in the malignant progression of colon cancer.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50

• UniProt:

  P12931

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 1-30 from the human protein was used as the immunogen for this c-Src antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, IF, WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the c-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This c-Src antibody is available for research use only.

• Storage Conditions:

  Aliquot the c-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the c-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of lysate from (1) human MCF-7, (2) rat PC-12, and (3) mouse C2C12 cell line using c-Src antibody at 1:1000.