Src Antibody

CAT: 0800-R31489Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R31489Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Sarcoma is a proto-oncogenic tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus. It belongs to a family of non-receptor tyrosine kinases called Src family kinases. The human proto-oncogene was assigned to chromosome 20. Its gene is mapped to 20q12-q13. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This proto-oncogene may play a role in the regulation of embryonic development and cell growth.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P12931
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 2-368) was used as the immunogen for this Src antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Src antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Src antibody is available for research use only.
Storage Conditions
After reconstitution, the Src antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, nuclear, cell membrane
Image Legend
IHC staining of FFPE human rectal cancer tissue with Src antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Product Datasheet
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