FGFR2 Antibody

• Description:

  FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.

• Specifications:

  IHC (Paraffin) : 1:25, Immunofluorescence: 1:25, Western blot: 1:1000

• UniProt:

  P21802

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A portion of amino acids 7-37 from the human protein was used as the immunogen for this FGFR2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, IF, WB, FACS, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the FGFR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This FGFR2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the FGFR2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the FGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of lysate from (1) HeLa, (2) K562 and (3) T47D cell line using FGFR2 antibody at 1:1000. Predicted molecular weight: 80-120 kDa. The observed size may be larger due to glycosylation.