ApoE Antibody (Apolipoprotein E)

• Description:

  Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

• UniProt:

  P02649

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 263-292 from the human protein was used as the immunogen for this ApoE antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the ApoE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This ApoE antibody is available for research use only.

• Storage Conditions:

  Aliquot the ApoE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the ApoE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Primate, Rabbit

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membranous

• Image Legend:

  Western blot analysis of lysate from human plasma tissue lysate using ApoE antibody diluted at 1:1000. Predicted molecular weight: 34-37 kDa