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Apolipoprotein E Antibody / APOE

CAT: 0800-RQ8075Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ8075Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Apolipoprotein E is also known as APOE, AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P02649
Host
Rabbit
Reactivity
Human
Immunogen
E. coli-derived recombinant human protein (amino acids E95-Q222) was used as the immunogen for the Apolipoprotein E antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Apolipoprotein E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Apolipoprotein E antibody is available for research use only.
Storage Conditions
After reconstitution, the Apolipoprotein E antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Apolipoprotein E antibody should be determined by the researcher.
Location
Cytoplasmic, membranous
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with Apolipoprotein E antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
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