Aurora-C Antibody

• Description:

  Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.

• Specifications:

  Western blot: 1:1000

• UniProt:

  Q9UQB9

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 3-38 from the human protein was used as the immunogen for this Aurora-C antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Aurora-C antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Aurora-C antibody is available for research use only.

• Storage Conditions:

  Aliquot the Aurora-C antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Aurora-C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Immunofluorescence staining of HeLa cells expressing GFP-Aurora-C performed at different cellular mitotic stages with the A) Aurora-C antibody, B) GFP fluorescence, C) DAPI nuclear staining, and D) anti-Aurora C merged to DAPI staining.