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IL-1 Antibody

CAT: 0800-F49920-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F49920-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL1A is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P01583
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 177-206 from human IL1A was used as the immunogen for this IL-1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Store at 4oC for up to one month. For long term, aliquot the IL-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IL-1 antibody is available for research use only.
Storage Conditions
Store at 4°C for up to one month. For long term, aliquot the IL-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the IL-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IL-1 antibody flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Product Datasheet
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