Phosphorylated-SOX2 Antibody (pS251)

• Description:

  This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT) .

• Specifications:

  Dot blot: 1:500

• UniProt:

  P48431

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This phosphorylated-SOX2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS251 of human SOX2.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  DB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the phosphorylated-SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This phosphorylated-SOX2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the phosphorylated-SOX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the phosphorylated-SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse

• CAS Number:

  9007-83-4

• Image Legend:

  Dot blot analysis of phosphorylated-Sox2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.