PHGDH Antibody

• Description:

  3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt:

  O43175

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A portion of amino acids 140-168 from the human protein was used as the immunogen for this PHGDH antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, FACS, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PHGDH antibody is available for research use only.

• Storage Conditions:

  Aliquot the PHGDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Bovine, Pig

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2) . Predicted molecular weight ~56 kDa.