CD19 Antibody

• Description:

  Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

• Specifications:

  Western blot: 1:500-100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

• UniProt:

  P15391

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 143-172 from the human protein was used as the immunogen for this CD19 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IF, FACS, IHC, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CD19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD19 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD19 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the CD19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Western blot analysis of lysate from Ramos cell line using CD19 antibody at 1:500. Expected size is 60~100 kDa depending on glycosylation level.