Anti-Src Antibody

• Description:

  This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:100-1000

• UniProt:

  P12931

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  This anti-Src antibody was produced from a mouse immunized with a full length recombinant human protein.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  17AT28

• Applications:

  WB, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the anti-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This anti-Src antibody is available for research use only.

• Storage Conditions:

  Aliquot the anti-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the anti-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of lysate from (1) HT29 and (2) Jurkat cell line using anti-Src antibody at 1:1000.