Anti-Src Antibody

• Description :

  This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

• Specifications :

  Immunofluorescence: 1:10-1:50, Western blot: 1:100-1000

• UniProt :

  P12931

• Host :

  Mouse

• Reactivity :

  Human, Mouse

• Immunogen :

  This anti-Src antibody was produced from a mouse immunized with a full length recombinant human protein.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  17AT28

• Applications :

  WB, IF, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the anti-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This anti-Src antibody is available for research use only.

• Storage Conditions :

  Aliquot the anti-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the anti-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of lysate from (1) HT29 and (2) Jurkat cell line using anti-Src antibody at 1:1000.