SOX2 Antibody

• Description:

  This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT) .

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:200-1:2000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt:

  P48431

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant protein was used to produce this monoclonal SOX2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  57CT23.3.4

• Applications:

  WB, IHC, IF, FACS, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This SOX2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the SOX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  Western blot analysis of lysate from NCCIT cell line using SOX2 antibody at 1:1000.