Α-Glucosidase (α-GC) Activity Assay Kit

• Target:

  Α-GC

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism

• Assay Principle:

  Α-Glucosidase (α-GC), also known as α-D-glucoside hydrolase, is widely distributed in nature, with a wide variety of species and different properties, and exists in almost all organisms. It has important physiological functions in carbohydrate metabolism in animals, plants and microorganisms. If α-GC is deficient, it leads to severe glycogen metabolism disorders and excessive glycogen accumulation, which can cause Pompah's disease (an autosomal recessive inherited glycogen storage disease, also known as glycogenosis type II) . Long-term low α-GC activity in human tissues can cause muscle fiber destruction and muscle atrophy. The detection principle of this kit is that the chromogenic substance generated by the substrate reaction catalyzed by α-glucosidase has the maximum absorption at 400 nm, and the enzyme activity of α-GC can be reflected by measuring its OD value at 400 nm.

• Assay Performance Time:

  50 min

• Sample Type:

  Tissue; fungus

• Detection Range:

  0.35-33.43 U/L

• Sensitivity:

  0.35 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  4.2-6.6%

• Intra CV:

  4.4-5.7%

• Detection Instrument:

  Microplate reader