Α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit

• Target:

  Α-KGDH

• Type:

  Colorimetric method

• Field of Research:

  Mitochondria Function; Tricarboxylic Acid (TCA) Cycle

• Assay Principle:

  Α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm

• Assay Performance Time:

  40 min

• Sample Type:

  Serum; plasma; animal; plant tissue

• Detection Range:

  8.3-42.3 U/L

• Sensitivity:

  8.3 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  8.5%

• Intra CV:

  1.5%

• Detection Instrument:

  Microplate reader