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AP2A1/AP2-Alpha Antibody

CAT: 0013-GTR18226210Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18226210Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AP2A1/AP2-Alpha Antibody
Product Name Alternative
AP2A1, Adaptin, alpha A, ADTAA, Alpha-adaptin A, Alpha1-adaptin, AP-2 complex subunit alpha-1, AP2 Alpha, CLAPA1, AP2-alpha
Reactivity
Canine, Equine, Human, Monkey, Mouse, Porcine, Rabbit
Immunogen
Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.
Target
AP2A1 / AP2-Alpha
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
ICC, IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2) . The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 ug/ml. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
Tested Applications
ELISA, ICC, IHC, IHC-P, WB
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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