Colon Lysate

CAT: 0223-21-102Size: 0.1 mgDry Ice: YesHazardous: No
CAT#:0223-21-102Size:0.1 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Bovine colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚ C. The bovine colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β -actin antibody, and the expression level is consistent with each lot.
Type
Lysates
Concentration
2 mg/mL or better
Buffer
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
Shipping Conditions
Dry Ice
Storage Conditions
Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Applications Notes
Colon lysate is for use in Western blotting, 10 μ g to 20 μ g per lane is recommended for mini gel.
Tested Applications
WB
Species
Bovine
Physical Properties
Liquid

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