Colon Tissue Lysate (Normal)

CAT: 0223-1715-22Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-1715-22Size:0.1 mg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μ g/ml of aprotinin, 5 μ g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β -mercaptoethanol.
Type
Lysates
Concentration
2 mg/mL
Buffer
Colon Tissue Lysate (Normal) is supplied in SDS sample buffer containing 5% β -mercaptoethanol.
Shipping Conditions
Blue Ice
Storage Conditions
Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Tested Applications
WB
Species
Human
Physical Properties
Liquid

Related Products

CatalogName
1716-03Colon Tissue Lysate (Tumor)
1716-04Colon Tissue Lysate (Tumor)
1716-02Colon Tissue Lysate (Tumor)
1716-01Colon Tissue Lysate (Tumor)
1710-08Breast Tissue Lysate (Normal)
1710-03Breast Tissue Lysate (Normal)
1710-04Breast Tissue Lysate (Normal)
1746-01Cervix Tissue Lysate (Normal)
1746-02Cervix Tissue Lysate (Normal)
1731-02Brain Tissue Lysate (Normal)

Alternative Products

CatalogName
MBS155031-01Colon Tissue Lysate (Normal)
NBP2-30177Colon Tissue Slides (Normal)
88Normal colon Tissue block
HAL-1320Human Adult Colon (Normal) Whole tissue lysate
MCL-1179Monkey (Cyno.) Normal Colon Whole tissue lysate
MRL-1288Monkey (Rhesus) Normal Colon Whole tissue lysate
C1434090cDNA - Rat Normal Tissue: Colon
C1334090cDNA - Mouse Normal Tissue: Colon
NBP2-47074-2VialColon Matched Tumor & Normal Tissue Lysate - (Dry Ice)

Popular Products