14-3-3 sigma Peptide

• NCBI Gene ID:

  2810

• Swiss Prot:

  P31947

• Accession Number:

  NP_006133.1

• Type:

  Peptides

• Buffer:

  14-3-3 sigma peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Store at -20˚ C. When reconstituted minimize freezing and thawing.

• Applications Notes:

  14-3-3 sigma Peptide is used for blocking the activity of 14-3-3 sigma antibody (catalog number 45-174) . The 14-3-3 sigma antibody ships at 0.5 mg/mL and the 14-3-3 sigma Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.

• NCBI Official Name:

  Stratifin

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  YWHAS, HME1, 14-3-3 protein sigma, Epithelial cell marker protein 1

• Tested Applications:

  Blocking

• Species:

  Human

• Protein ID:

  5454052

• Physical Properties:

  Lyophilized