VEGF R1 / Flt-1 Recombinant Protein

• Background:

  Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

• NCBI Gene ID:

  14254

• Swiss Prot:

  P35969

• Accession Number:

  NP_034358.2

• Tag:

  Mouse IgG Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Immunology, Obesity, Signal Transduction, Stem Cell

• Purity:

  >85% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 100 mM Glycine, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  109.8 kDa

• Applications Notes:

  This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 109.8 kDa. The protein migrates as 140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  FMS-like tyrosine kinase 1

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Shibuya M., et al., 1990, Oncogene 5:519-524.

• Background Reference 02:

  Kendall R.L., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:10705-10709.

• Background Reference 03:

  Seetharam L., et al., 1995, Oncogene 10:135-147.

• Background Reference 04:

  Barleon B., et al., 1996, Blood 87:3336-3343.

• Other Product Names:

  FLT, VEGFR1, FLT1

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3