SIRPA Recombinant Protein

• Background:

  Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

• NCBI Gene ID:

  140885

• Swiss Prot:

  P78324

• Accession Number:

  NP_001035111

• Tag:

  C-mFc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Signal Transduction

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  50 mM tris, 100 mM glycine, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  63.5 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  Signal-regulatory protein alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Timms J.F., et al., 1999, Curr. Biol. 9:927-930.

• Background Reference 02:

  Stofega M.R., et al., 2000, J. Biol. Chem. 275:28222-28229.

• Background Reference 03:

  Latour S., et al., 2001, J. Immunol. 167:2547-2554.

• Other Product Names:

  SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3