Integrin alpha 4 Recombinant Protein

• Background:

  The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains (reviewed in 1) . Alpha 4 (4) chain associates with either beta 1 (1) or beta 77) chain. It has been demonstrated that the putative ligand-binding sites of both integrin 41 and 47 is located on the 4 chain. These ligands included Madcam, VCAM, and fibronectin (2-4) . Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin 47, the gut mucosal homing receptor for peripheral T cells (5) . This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 (IN1) (PSI 4827) and Integrin 4 (IN2) (PSI 4837), but not Integrin 4 (CT) (PSI 4783) .

• NCBI Gene ID:

  3676

• Swiss Prot:

  P13612

• Accession Number:

  NP_000876

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Immunology, Infectious Disease, Stem Cell

• Purity:

  ~95%

• Buffer:

  50mM Tris pH7.5, 150mM NaCl, 5 mM MgCl2

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  17 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol. 2008; 25:376-87.

• Background Reference 02:

  Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29) . Biochem J. 1995; 305:945-51.

• Background Reference 03:

  Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 1993; 74:185-95.

• Background Reference 04:

  Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem. 1991; 266:10241-5.

• Other Product Names:

  Integrin alpha 4 Antibody: IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Protein ID:

  67191027

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3