ENPP3 / CD203c Recombinant Protein

• Background:

  The human NPP family contains seven members which can be classified into two groups according to their substrate preferences. The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4. NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells. Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface. This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry. Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions. Following activation, these cells release ATP, which further stimulates them in an autocrine manner. NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response) .

• NCBI Gene ID:

  5169

• Swiss Prot:

  O14638

• Accession Number:

  NP_005012.2

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  50 mM Tris, 150 mM NaCl, pH8.0

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  96.9 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 96.9 kDa. The protein migrates as 115-120 kDa and 121-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Ectonucleotidepyrophosphatase/phosphodiesterase3

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  ENPP3, CD203c, NPP3, E-NPP3, PD-Ibeta, NPPase, PDNP3

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3