LGR4 / GPR48 Recombinant Protein

• Background:

  The fourth member of the leucine-rich repeat-containing GPCR family (LGR4, frequently referred to as GPR48) together with its family members LGR5 and -6, bind to R-spondins (RSPOs) -1–4 and result in Wnt signaling potentiation. In addition, LGR4 (as well as LGR5 and -6) is implicated in multiple cancers and promotes invasion and metastasis in colorectal, prostate, and cervical cancer cell lines.

• NCBI Gene ID:

  55366

• Swiss Prot:

  Q9BXB1

• Accession Number:

  NP_060960.2

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >85% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  58.8 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.8 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  LeucinerichrepeatcontainingGprotein-coupledreceptor4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yue Z. et al. FASEB J. 2018. 32 (5) :2422-2437.

• Background Reference 02:

  Gao Y. et al. Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis. Cancer Res. 2006 Dec 15; 66 (24) :11623-31.

• Background Reference 03:

  Gao Y. et al. 2009. Chin. J. Cancer 28, 104–107.

• Background Reference 04:

  Liang F. et al. 2015. Med Oncol. 2015 Mar;32 (3) :49.

• Other Product Names:

  LGR4, GPR48, G-protein coupled receptor 48, Leucine-rich repeat-containing G-protein coupled receptor 4

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3