Rhesus macaque CD28 Recombinant Protein

• Background:

  T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

• NCBI Gene ID:

  940

• Swiss Prot:

  P10747

• Accession Number:

  NP_006130.1

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Homeostasis, Immunology, Obesity, Signal Transduction, Stem Cell

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  41.6 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  CD28 molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Evans E.J., et al., 2005, Nat. Immunol. 6:271-279.

• Background Reference 02:

  Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46.

• Background Reference 03:

  Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.

• Other Product Names:

  CD28, Tp44

• Tested Applications:

  ELISA, WB

• Species:

  Human, Cynomolgus, Rhesus macaque

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3