Rhesus macaque CD47 Recombinant Protein

• Background:

  Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

• NCBI Gene ID:

  704980

• Accession Number:

  NP_001253446.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Cancer, Immunology, Neuroscience

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  15.8 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 30-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  CD47 molecule

• NCBI Organism:

  Macaca mulatta

• Background Reference 01:

  Lindberg F.P., et al., 1993, J. Cell Biol. 123:485-496.

• Background Reference 02:

  Latour S., et al., 2001, J. Immunol. 167:2547-2554.

• Background Reference 03:

  Piccio L., et al., 2005, Blood 105:2421-2427.

• Other Product Names:

  CD47, MER6, IAP, OA3

• Tested Applications:

  ELISA, WB

• Species:

  Cynomolgus, Rhesus macaque

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3