Siglec-8 Recombinant Protein

• Background:

  Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8. Siglec-8 was first identified by CD33 homology screening of ESTs from a cDNA library generated from a patient diagnosed with idiopathic hypereosinophilic syndrome and was originally termed SAF-2 (sialoadhesin family 2) . At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Two splice variants of Siglec-8 exist. The initially characterized form contains 431 amino acid residues in total, subsequently, a longer form of Siglec-8, initially termed Siglec-8L. Both forms of Siglec-8 are found in eosinophils and contain a V-set domain with lectin activity and two C2-type Ig repeat domains in the extracellular region.

• NCBI Gene ID:

  27181

• Swiss Prot:

  Q9NYZ4

• Accession Number:

  NP_055257.2

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  64.3 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.3 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  SialicacidbindingIglikelectin8

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Floyd H, et al. 2000. J Biol Chem. 275 (2) :861-6.

• Background Reference 02:

  Foussias G, et al. 2000. Biochem Biophys Res Commun. 278 (3) :775-81.

• Background Reference 03:

  Kikly KK, et al. 2000. J Allergy Clin Immunol. 105 (6 Pt 1) :1093-100.

• Other Product Names:

  Siglec-8, SAF-2, SIGLEC8, SAF2

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3