PDL1 Recombinant Protein

• Background:

  Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

• NCBI Gene ID:

  60533

• Swiss Prot:

  Q9EP73

• Accession Number:

  NP_068693

• Tag:

  C-Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >92% as determined by SDS-PAGE.

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  51.4 kDa

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• NCBI Official Name:

  CD274 antigen

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Dong H., et al., 1999, Nat. Med. 5:1365-1369.

• Background Reference 02:

  Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.

• Background Reference 03:

  Nishimura H., et al., 2001, Trends in Immunology 22:265.

• Other Product Names:

  PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

• Tested Applications:

  WB

• Species:

  Mouse

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3