PDL1 Antibody [1F11]

• Background:

  PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2) . PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3) . This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4) .

• NCBI Gene ID:

  29126

• Swiss Prot:

  Q9NZQ7

• Accession Number:

  NP_054862

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  PD-L1 antibody was raised against the extracellular domain of human PD-L1.

• Clonality:

  Monoclonal

• Clone:

  1F11

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  PD-L1 Antibody is supplied as protein A purified IgG1.

• Positive Control:

  Cat. No. 1340 - Human Stomach Carcinoma Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PD-L1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG1

• Symbol:

  CD274

• Positive Control 2:

  Cat. No. 1315 - Human Tonsil Tissue

• NCBI Official Name:

  Programmed cell death 1 ligand

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.

• Background Reference 02:

  Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.

• Background Reference 03:

  LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1) . Hum. Immunol. 2006; 67:568-78.

• Background Reference 04:

  Aydin AM, Woldu SL, Hutchinson RC, et al. Spotlight on atezolizumab and its potential in the treatment of advanced urothelial bladder cancer.Onco. Targets Ther. 2017;10:1487-502.

• Other Product Names:

  PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1

• Tested Applications:

  ELISA, WB, IHC-P, ICC, IF

• Protein ID:

  7661534

• Physical Properties:

  Liquid